HPV16 methyl-haplotypes determined by a novel next-generation sequencing method are associated with cervical precancer

We have developed and evaluated a next-generation bisulfite sequencing (NGS) assay to distinguish HPV16 cervical precancer (CIN2-3; N=59) from HPV16-positive transient infections (N=40). Cervical DNA was isolated and treated with bisulfite and HPV16 methylation was quantified by (1) amplification wi...

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Detalhes bibliográficos
Publicado no:Int J Cancer
Main Authors: Mirabello, Lisa, Frimer, Marina, Harari, Ariana, McAndrew, Thomas, Smith, Benjamin, Chen, Zigui, Wentzensen, Nicolas, Wacholder, Sholom, Castle, Philip E., Raine-Bennett, Tina, Schiffman, Mark, Burk, Robert D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4262737/
https://ncbi.nlm.nih.gov/pubmed/25081507
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/ijc.29119
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