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Transfer of Liposome-Sequestering Plasmid DNA into Daucus carota Protoplasts

Reverse-phase evaporation lipid vesicles (REV) liposomes, consisting of phosphatidyl choline and stearylamine in 1:3 molar ratio, encapsulated approximately 30% of exogenously supplied recombinant DNA vector, pBR322. The DNA sequestered in REV liposomes was highly tolerant to DNase. A two-step proce...

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Bibliografiske detaljer
Main Authors: Uchimiya, Hirofumi, Harada, Hiroshi
Format: Artigo
Sprog:Inglês
Udgivet: 1981
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC426039/
https://ncbi.nlm.nih.gov/pubmed/16662045
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