Comparison of Whole Blood RNA Preservation Tubes and Novel Generation RNA Extraction Kits for Analysis of mRNA and MiRNA Profiles

BACKGROUND: Whole blood expression profiling is frequently performed using PAXgene (Qiagen) or Tempus (Life Technologies) tubes. Here, we compare 6 novel generation RNA isolation protocols with respect to RNA quantity, quality and recovery of mRNA and miRNA. METHODS: 3 PAXgene and 3 Tempus Tubes wer...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Häntzsch, Madlen, Tolios, Alexander, Beutner, Frank, Nagel, Dorothea, Thiery, Joachim, Teupser, Daniel, Holdt, Lesca M.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4254602/
https://ncbi.nlm.nih.gov/pubmed/25469788
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0113298
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