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Efficient Cryoprotection of Macromolecular Crystals using Vapor Diffusion of Volatile Alcohols

Macromolecular X-ray crystallography, usually done at cryogenic temperature to limit radiation damage, often requires liquid cryoprotective soaking that can be labor intensive and damaging to crystals. Here we describe a method for cryoprotection that uses vapor diffusion of volatile cryoprotective...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:J Struct Biol
Main Authors: Farley, Christopher, Juers, Douglas H.
Format: Artigo
Jezik:Inglês
Izdano: 2014
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4252874/
https://ncbi.nlm.nih.gov/pubmed/25286441
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2014.09.011
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