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Successful Transient Expression of Cas9 and Single Guide RNA Genes in Chlamydomonas reinhardtii
The clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system has become a powerful and precise tool for targeted gene modification (e.g., gene knockout and gene replacement) in numerous eukaryotic organisms. Initial attempts to apply this technology to a model, the single-cell a...
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| Publicado en: | Eukaryot Cell |
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| Autores principales: | , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Microbiology
2014
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4248704/ https://ncbi.nlm.nih.gov/pubmed/25239977 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/EC.00213-14 |
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