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Successful Transient Expression of Cas9 and Single Guide RNA Genes in Chlamydomonas reinhardtii

The clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system has become a powerful and precise tool for targeted gene modification (e.g., gene knockout and gene replacement) in numerous eukaryotic organisms. Initial attempts to apply this technology to a model, the single-cell a...

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Detalhes bibliográficos
Publicado no:Eukaryot Cell
Main Authors: Jiang, Wenzhi, Brueggeman, Andrew J., Horken, Kempton M., Plucinak, Thomas M., Weeks, Donald P.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4248704/
https://ncbi.nlm.nih.gov/pubmed/25239977
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/EC.00213-14
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