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Tn5 transposase and tagmentation procedures for massively scaled sequencing projects

Massively parallel DNA sequencing of thousands of samples in a single machine-run is now possible, but the preparation of the individual sequencing libraries is expensive and time-consuming. Tagmentation-based library construction, using the Tn5 transposase, is efficient for generating sequencing li...

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Detalhes bibliográficos
Publicado no:Genome Res
Main Authors: Picelli, Simone, Björklund, Åsa K., Reinius, Björn, Sagasser, Sven, Winberg, Gösta, Sandberg, Rickard
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4248319/
https://ncbi.nlm.nih.gov/pubmed/25079858
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.177881.114
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