Expansion of the genetic toolkit for metabolic engineering of Clostridium pasteurianum: chromosomal gene disruption of the endogenous CpaAI restriction enzyme

BACKGROUND: Clostridium pasteurianum is one of the most promising biofuel producers within the genus Clostridium owing to its unique metabolic ability to ferment glycerol into butanol. Although an efficient means is available for introducing foreign DNA to C. pasteurianum, major genetic tools, such...

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Publicado en:Biotechnol Biofuels
Autores principales: Pyne, Michael E, Moo-Young, Murray, Chung, Duane A, Chou, C Perry
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2014
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Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4245778/
https://ncbi.nlm.nih.gov/pubmed/25431621
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13068-014-0163-1
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