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A 3D Time-Shared NOESY Experiment Designed to Provide Optimal Resolution for Accurate Assignment of NMR Distance Restraints in Large Proteins

Structure determination of proteins by solution NMR has become an established method, but challenges increase steeply with the size of proteins. Notably spectral crowding and signal overlap impair the analysis of cross-peaks in NOESY spectra that provide distance restraints for structural models. An...

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Pubblicato in:J Biomol NMR
Autori principali: Mishra, Subrata H, Harden, Bradley J, Frueh, Dominique P
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2014
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4245328/
https://ncbi.nlm.nih.gov/pubmed/25381567
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-014-9873-8
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