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Use of the Meganuclease I-SceI of Saccharomyces cerevisiae to select for gene deletions in actinomycetes

The search for new natural products is leading to the isolation of novel actinomycete species, many of which will ultimately require genetic analysis. Some of these isolates will likely exhibit low intrinsic frequencies of homologous recombination and fail to sporulate under laboratory conditions, e...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Fernández-Martínez, Lorena T., Bibb, Mervyn J.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4235310/
https://ncbi.nlm.nih.gov/pubmed/25403842
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep07100
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