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Use of the Meganuclease I-SceI of Saccharomyces cerevisiae to select for gene deletions in actinomycetes

The search for new natural products is leading to the isolation of novel actinomycete species, many of which will ultimately require genetic analysis. Some of these isolates will likely exhibit low intrinsic frequencies of homologous recombination and fail to sporulate under laboratory conditions, e...

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Bibliografische gegevens
Gepubliceerd in:Sci Rep
Hoofdauteurs: Fernández-Martínez, Lorena T., Bibb, Mervyn J.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Nature Publishing Group 2014
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4235310/
https://ncbi.nlm.nih.gov/pubmed/25403842
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep07100
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