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Crystallization and preliminary X-ray crystallographic analysis of an artificial molten-globular-like triosephosphate isomerase protein of mixed phylogenetic origin

A bioinformatics-based protein-engineering approach called consensus design led to the construction of a chimeric triosephosphate isomerase (TIM) protein called ccTIM (curated consensus TIM) which is as active as Saccharomyces cerevisiae TIM despite sharing only 58% sequence identity with it. The am...

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Detalhes bibliográficos
Publicado no:Acta Crystallogr F Struct Biol Commun
Main Authors: Goyal, Venuka Durani, Yadav, Pooja, Kumar, Ashwani, Ghosh, Biplab, Makde, Ravindra D.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4231856/
https://ncbi.nlm.nih.gov/pubmed/25372821
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X14020755
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