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CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis

Knockout of genes with CRISPR/Cas9 is a newly emerged approach to investigate functions of genes in various organisms. We demonstrate that CRISPR/Cas9 can mutate endogenous genes of the ascidian Ciona intestinalis, a splendid model for elucidating molecular mechanisms for constructing the chordate b...

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Bibliografiske detaljer
Udgivet i:Dev Growth Differ
Main Authors: Sasaki, Haruka, Yoshida, Keita, Hozumi, Akiko, Sasakura, Yasunori
Format: Artigo
Sprog:Inglês
Udgivet: BlackWell Publishing Ltd 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4231237/
https://ncbi.nlm.nih.gov/pubmed/25212715
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/dgd.12149
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