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NADH-dependent biosensor in Saccharomyces cerevisiae: principle and validation at the single cell level

A reporter system was constructed to measure perturbations in the NADH/NAD(+) co-factor balance in yeast, by using the green fluorescent protein gene under the control of the GPD2 promoter that is induced under conditions of excess of NADH. High fluorescence levels were obtained in a glycerol 3-phos...

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Bibliografische gegevens
Gepubliceerd in:AMB Express
Hoofdauteurs: Knudsen, Jan Dines, Carlquist, Magnus, Gorwa-Grauslund, Marie
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Springer 2014
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4230897/
https://ncbi.nlm.nih.gov/pubmed/25401080
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13568-014-0081-4
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