Virulence of Shigella flexneri Hybrids Expressing Escherichia coli Somatic Antigens

The genes controlling either Escherichia coli somatic antigen 8 or 25 were conjugally transferred to virulent Shigella flexneri 2a recipients to determine whether the aquisition of these antigens would affect the virulence of the resulting hybrid. A high proportion of such hybrids were found to be r...

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Główni autorzy: Gemski, P., Sheahan, D. G., Washington, O., Formal, S. B.
Format: Artigo
Język:Inglês
Wydane: 1972
Hasła przedmiotowe:
Pathogenic Mechanisms, Ecology, and Epidemiology
Dostęp online:https://ncbi.nlm.nih.gov/pmc/articles/PMC422501/
https://ncbi.nlm.nih.gov/pubmed/4569915
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spelling pubmed-4225012004-10-06 Virulence of Shigella flexneri Hybrids Expressing Escherichia coli Somatic Antigens Gemski, P. Sheahan, D. G. Washington, O. Formal, S. B. Infect Immun Pathogenic Mechanisms, Ecology, and Epidemiology The genes controlling either Escherichia coli somatic antigen 8 or 25 were conjugally transferred to virulent Shigella flexneri 2a recipients to determine whether the aquisition of these antigens would affect the virulence of the resulting hybrid. A high proportion of such hybrids were found to be rough and hence were avirulent. Some smooth S. flexneri hybrids which replaced their native group antigens with E. coli factor 25 were still virulent in the animal models employed. All S. flexneri O-8 hybrids were uniformly avirulent. Our finding, that S. flexneri hybrids with the chemically divergent E. coli O-8 repeat unit are avirulent whereas some hybrids with the chemically related O-25 repeat unit retain virulence, suggests that the chemical composition and structure of the O side chain of somatic antigens may represent one determining factor for bacterial penetration of mucosal epithelial cells, the primary step in the pathogenesis of bacillary dysentery. 1972-08 /pmc/articles/PMC422501/ /pubmed/4569915 Text en Copyright © 1972 American Society for Microbiology
institution US NLM
collection PubMed Central
language Inglês
format Artigo
topic Pathogenic Mechanisms, Ecology, and Epidemiology
spellingShingle Pathogenic Mechanisms, Ecology, and Epidemiology
Gemski, P.
Sheahan, D. G.
Washington, O.
Formal, S. B.
Virulence of Shigella flexneri Hybrids Expressing Escherichia coli Somatic Antigens
description The genes controlling either Escherichia coli somatic antigen 8 or 25 were conjugally transferred to virulent Shigella flexneri 2a recipients to determine whether the aquisition of these antigens would affect the virulence of the resulting hybrid. A high proportion of such hybrids were found to be rough and hence were avirulent. Some smooth S. flexneri hybrids which replaced their native group antigens with E. coli factor 25 were still virulent in the animal models employed. All S. flexneri O-8 hybrids were uniformly avirulent. Our finding, that S. flexneri hybrids with the chemically divergent E. coli O-8 repeat unit are avirulent whereas some hybrids with the chemically related O-25 repeat unit retain virulence, suggests that the chemical composition and structure of the O side chain of somatic antigens may represent one determining factor for bacterial penetration of mucosal epithelial cells, the primary step in the pathogenesis of bacillary dysentery.
author Gemski, P.
Sheahan, D. G.
Washington, O.
Formal, S. B.
author_facet Gemski, P.
Sheahan, D. G.
Washington, O.
Formal, S. B.
author_sort Gemski, P.
title Virulence of Shigella flexneri Hybrids Expressing Escherichia coli Somatic Antigens
title_short Virulence of Shigella flexneri Hybrids Expressing Escherichia coli Somatic Antigens
title_full Virulence of Shigella flexneri Hybrids Expressing Escherichia coli Somatic Antigens
title_fullStr Virulence of Shigella flexneri Hybrids Expressing Escherichia coli Somatic Antigens
title_full_unstemmed Virulence of Shigella flexneri Hybrids Expressing Escherichia coli Somatic Antigens
title_sort virulence of shigella flexneri hybrids expressing escherichia coli somatic antigens
publishDate 1972
url https://ncbi.nlm.nih.gov/pmc/articles/PMC422501/
https://ncbi.nlm.nih.gov/pubmed/4569915
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