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Total chemical synthesis of a ribozyme derived from a group I intron.

We describe the complete chemical synthesis of a ribozyme that catalyzes template-directed oligonucleotide ligation. The specific activity of the synthetic ribozyme is nearly identical to that of the same enzyme generated by in vitro transcription with T7 RNA polymerase. The ribozyme is derived from...

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Publicado en:Proc Natl Acad Sci U S A
Autores principales: Whoriskey, S K, Usman, N, Szostak, J W
Formato: Artigo
Lenguaje:Inglês
Publicado: National Academy of Sciences 1995
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Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC42238/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7708666/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.92.7.2465
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