Molecular Flow Quantified beyond the Diffraction Limit by Spatiotemporal Image Correlation of Structured Illumination Microscopy Data

We combine total internal reflection fluorescence structured illumination microscopy with spatiotemporal image correlation spectroscopy to quantify the flow velocities and directionality of filamentous-actin at the T cell immunological synapse. These techniques demonstrate it is possible to image re...

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Detalles Bibliográficos
Publicado en:Biophys J
Main Authors: Ashdown, George W., Cope, Andrew, Wiseman, Paul W., Owen, Dylan M.
Formato: Artigo
Idioma:Inglês
Publicado: The Biophysical Society 2014
Assuntos:
Biophysical Letter
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4223199/
https://ncbi.nlm.nih.gov/pubmed/25418107
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2014.09.018
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