Succinimidyl Ester Surface Chemistry: Implications of the Competition between Aminolysis and Hydrolysis on Covalent Protein Immobilization

[Image: see text] N-Hydroxysuccinimide (NHS) ester terminal groups are commonly used to covalently couple amine-containing biomolecules (e.g., proteins and peptides) to surfaces via amide linkages. This one-step aminolysis is often performed in buffered aqueous solutions near physiological pH (pH 6...

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Detalhes bibliográficos
Publicado no:Langmuir
Main Authors: Lim, China Y., Owens, Nicholas A., Wampler, Ronald D., Ying, Yixin, Granger, Jennifer H., Porter, Marc D., Takahashi, Makoto, Shimazu, Katsuaki
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2014
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4222659/
https://ncbi.nlm.nih.gov/pubmed/25317495
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/la503439g
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