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Design and Analysis of Hammerhead Ribozyme Activity Against an Artificial Gene Target
In vitro cleavage assays are routinely conducted to properly assess the catalytic activity of hammerhead ribozymes (HHR) against target RNA molecules like the dengue virus RNA genomes. These experiments are performed for initial assessment of HHR catalysis in a cell-free system and have been simplif...
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| Publicado no: | Methods Mol Biol |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4219533/ https://ncbi.nlm.nih.gov/pubmed/24318886 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-730-3_5 |
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