Metal Binding by the D,DX(35)E Motif of Human Immunodeficiency Virus Type 1 Integrase: Selective Rescue of Cys Substitutions by Mn(2+) In Vitro

The D,DX(35)E motif characteristic of retroviral integrase enzymes (INs) is expected to bind the required metal cofactors (Mg(2+) or Mn(2+)), but direct evidence for a catalytic role has been lacking. Here we used a metal rescue strategy to investigate metal binding. We established conditions for an...

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Bibliografische gegevens

Gepubliceerd in:	J Virol
Hoofdauteurs:	Gao, Kui, Wong, Steven, Bushman, Frederic
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	American Society for Microbiology (ASM) 2004
Onderwerpen:	Replication
Online toegang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC421655/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15194746/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JVI.78.13.6715-6722.2004
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