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Antibody-Free Magnetic Cell Sorting of Genetically Modified Primary Human CD4+ T Cells by One-Step Streptavidin Affinity Purification

Existing methods for phenotypic selection of genetically modified mammalian cells suffer disadvantages of time, cost and scalability and, where antibodies are used to bind exogenous cell surface markers for magnetic selection, typically yield cells coated with antibody-antigen complexes and beads. T...

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Библиографические подробности
Главные авторы: Matheson, Nicholas J., Peden, Andrew A., Lehner, Paul J.
Формат: Artigo
Язык:Inglês
Опубликовано: Public Library of Science 2014
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4216076/
https://ncbi.nlm.nih.gov/pubmed/25360777
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0111437
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