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DNA polymerases engineered by directed evolution to incorporate non-standard nucleotides

DNA polymerases have evolved for billions of years to accept natural nucleoside triphosphate substrates with high fidelity and to exclude closely related structures, such as the analogous ribonucleoside triphosphates. However, polymerases that can accept unnatural nucleoside triphosphates are desire...

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Dettagli Bibliografici
Autori principali: Laos, Roberto, Thomson, J. Michael, Benner, Steven A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Frontiers Media S.A. 2014
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4215692/
https://ncbi.nlm.nih.gov/pubmed/25400626
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2014.00565
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