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Effect of RNA integrity on uniquely mapped reads in RNA-Seq
BACKGROUND: We examined the performance of three RNA-Sequencing library preparation protocols as a function of RNA integrity, comparing gene expressions between heat-degraded samples to their high-quality counterparts. This work is invaluable given the difficulty of obtaining high-quality RNA from t...
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| Главные авторы: | , , , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
BioMed Central
2014
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4213542/ https://ncbi.nlm.nih.gov/pubmed/25339126 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-7-753 |
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