Effect of RNA integrity on uniquely mapped reads in RNA-Seq

BACKGROUND: We examined the performance of three RNA-Sequencing library preparation protocols as a function of RNA integrity, comparing gene expressions between heat-degraded samples to their high-quality counterparts. This work is invaluable given the difficulty of obtaining high-quality RNA from t...

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Bibliographic Details
Main Authors: Chen, Emily A, Souaiaia, Tade, Herstein, Jennifer S, Evgrafov, Oleg V, Spitsyna, Valeria N, Rebolini, Danea F, Knowles, James A
Format: Artigo
Language:Inglês
Published: BioMed Central 2014
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Correspondence
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4213542/
https://ncbi.nlm.nih.gov/pubmed/25339126
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-7-753
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