Construction of recombinant eukaryotic expression plasmid containing murine CD40 ligand gene and its expression in H22 cells

AIM: To construct a recombinant murine CD40 ligand (mCD40L) eukaryotic expression vector for gene therapy and target therapy of hepatocellular carcinoma (HCC). METHODS: mCD40L cDNA was synthesized by RT-PCR with the specific primers and directly cloned into T vector to generate middle recombinant. A...

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Detalhes bibliográficos
Main Authors: Jiang, Yong-Fang, He, Yan, Gong, Guo-Zhong, Chen, Jun, Yang, Chun-Yan, Xu, Yun
Formato: Artigo
Idioma:Inglês
Publicado em: Baishideng Publishing Group Co., Limited 2005
Assuntos:
Liver Cancer
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4205398/
https://ncbi.nlm.nih.gov/pubmed/15633212
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v11.i2.182
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