Construction of a recombinant eukaryotic expression vector containing a leptin gene and its expression in HPMSCs

Leptin gene fragments were amplified from human adipose tissue using reverse transcription polymerase chain reaction technology. The leptin gene was reconstructed in pIRES2-EGFP and transfected into human placenta-derived mesenchymal stem cells (HPMSCs) using a liposome-mediated method. Leptin mRNA...

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Bibliografiske detaljer
Main Authors: Jin, Julou, Wang, Bowei, Zhu, Zhenwei, Chen, Yifu, Mao, Zhi, Wang, Jinglong, Du, Bing, Wang, Shuping, Liu, Zhihui
Format: Artigo
Sprog:Inglês
Udgivet: Springer Netherlands 2013
Fag:
Original Research
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3973795/
https://ncbi.nlm.nih.gov/pubmed/23807594
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-013-9599-6
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