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Construction of a recombinant eukaryotic expression vector containing a leptin gene and its expression in HPMSCs
Leptin gene fragments were amplified from human adipose tissue using reverse transcription polymerase chain reaction technology. The leptin gene was reconstructed in pIRES2-EGFP and transfected into human placenta-derived mesenchymal stem cells (HPMSCs) using a liposome-mediated method. Leptin mRNA...
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Main Authors: | , , , , , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Springer Netherlands
2013
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3973795/ https://ncbi.nlm.nih.gov/pubmed/23807594 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-013-9599-6 |
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