Mutational analysis of the conserved region 2 site of adenovirus E1A and its effect on binding to the retinoblastoma gene product: use of the "double-tagging" assay.
We have explored the feasibility of using a "double-tagging" assay for assessing which amino acids of a protein are responsible for its binding to another protein. We have chosen the adenovirus E1A-retinoblastoma gene product (pRB) proteins for a model system, and we focused on the high-affinity con...
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| Publicado en: | Proc Natl Acad Sci U S A |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
1995
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC41998/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7753854/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.92.10.4631 |
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