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Freezing in Sealed Capillaries for Preparation of Frozen Hydrated Sections

We have investigated the freezing of specimens in a confined volume for preparation of vitreous samples for cryosectioning. With 15% dextran as a cryoprotectant, a sample sealed in a copper tube begins to freeze into crystalline ice when plunged into liquid ethane. Crystallization rapidly causes an...

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Détails bibliographiques
Auteurs principaux: Yakovlev, Sergey, Downing, Kenneth H.
Format: Artigo
Langue:Inglês
Publié: 2011
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4199587/
https://ncbi.nlm.nih.gov/pubmed/22077543
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1365-2818.2011.03575.x
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