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Freezing in Sealed Capillaries for Preparation of Frozen Hydrated Sections
We have investigated the freezing of specimens in a confined volume for preparation of vitreous samples for cryosectioning. With 15% dextran as a cryoprotectant, a sample sealed in a copper tube begins to freeze into crystalline ice when plunged into liquid ethane. Crystallization rapidly causes an...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4199587/ https://ncbi.nlm.nih.gov/pubmed/22077543 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1365-2818.2011.03575.x |
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