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Viewing Protein Fitness Landscapes Through a Next-Gen Lens

High-throughput sequencing has enabled many powerful approaches in biological research. Here, we review sequencing approaches to measure frequency changes within engineered mutational libraries subject to selection. These analyses can provide direct estimates of biochemical and fitness effects for a...

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Detalhes bibliográficos
Main Authors: Boucher, Jeffrey I., Cote, Pamela, Flynn, Julia, Jiang, Li, Laban, Aneth, Mishra, Parul, Roscoe, Benjamin P., Bolon, Daniel N. A.
Formato: Artigo
Idioma:Inglês
Publicado em: Genetics Society of America 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4196605/
https://ncbi.nlm.nih.gov/pubmed/25316787
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.114.168351
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