Direct purification of detergent-insoluble membranes from Medicago truncatula root microsomes: comparison between floatation and sedimentation

BACKGROUND: Membrane microdomains are defined as highly dynamic, sterol- and sphingolipid-enriched domains that resist to solubilization by non-ionic detergents. In plants, these so-called Detergent Insoluble Membrane (DIM) fractions have been isolated from plasma membrane by using conventional ultr...

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Detalhes bibliográficos
Main Authors: Guillier, Christelle, Cacas, Jean-Luc, Recorbet, Ghislaine, Deprêtre, Nicolas, Mounier, Arnaud, Mongrand, Sébastien, Simon-Plas, Françoise, Wipf, Daniel, Dumas-Gaudot, Eliane
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2014
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4193990/
https://ncbi.nlm.nih.gov/pubmed/25267185
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12870-014-0255-x
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