Optimization of iTRAQ labelling coupled to OFFGEL fractionation as a proteomic workflow to the analysis of microsomal proteins of Medicago truncatula roots

BACKGROUND: Shotgun proteomics represents an attractive technical framework for the study of membrane proteins that are generally difficult to resolve using two-dimensional gel electrophoresis. The use of iTRAQ, a set of amine-specific isobaric tags, is currently the labelling method of choice allow...

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Autors principals: Abdallah, Cosette, Sergeant, Kjell, Guillier, Christelle, Dumas-Gaudot, Eliane, Leclercq, Céline C, Renaut, Jenny
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2012
Matèries:
Research
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3442994/
https://ncbi.nlm.nih.gov/pubmed/22672774
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1477-5956-10-37
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