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Improved Genome Editing in Human Cell Lines Using the CRISPR Method
The Cas9/CRISPR system has become a popular choice for genome editing. In this system, binding of a single guide (sg) RNA to a cognate genomic sequence enables the Cas9 nuclease to induce a double-strand break at that locus. This break is next repaired by an error-prone mechanism, leading to mutatio...
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| 主要な著者: | , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Public Library of Science
2014
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4193831/ https://ncbi.nlm.nih.gov/pubmed/25303670 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0109752 |
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