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False Positives in Multiplex PCR-Based Next-Generation Sequencing Have Unique Signatures
Next-generation sequencing shows great promise by allowing rapid mutational analysis of multiple genes in human cancers. Recently, we implemented the multiplex PCR-based Ion AmpliSeq Cancer Hotspot Panel (>200 amplicons in 50 genes) to evaluate EGFR, KRAS, and BRAF in lung and colorectal adenocar...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Investigative Pathology
2014
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4188281/ https://ncbi.nlm.nih.gov/pubmed/25017478 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmoldx.2014.06.001 |
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