False Positives in Multiplex PCR-Based Next-Generation Sequencing Have Unique Signatures

Next-generation sequencing shows great promise by allowing rapid mutational analysis of multiple genes in human cancers. Recently, we implemented the multiplex PCR-based Ion AmpliSeq Cancer Hotspot Panel (>200 amplicons in 50 genes) to evaluate EGFR, KRAS, and BRAF in lung and colorectal adenocar...

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Bibliografiske detaljer
Main Authors: McCall, Chad M., Mosier, Stacy, Thiess, Michele, Debeljak, Marija, Pallavajjala, Aparna, Beierl, Katie, Deak, Kristen L., Datto, Michael B., Gocke, Christopher D., Lin, Ming-Tseh, Eshleman, James R.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Investigative Pathology 2014
Fag:
Regular Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4188281/
https://ncbi.nlm.nih.gov/pubmed/25017478
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmoldx.2014.06.001
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