Hybridization Capture Using Short PCR Products Enriches Small Genomes by Capturing Flanking Sequences (CapFlank)

Solution hybridization capture methods utilize biotinylated oligonucleotides as baits to enrich homologous sequences from next generation sequencing (NGS) libraries. Coupled with NGS, the method generates kilo to gigabases of high confidence consensus targeted sequence. However, in many experiments,...

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Main Authors: Tsangaras, Kyriakos, Wales, Nathan, Sicheritz-Pontén, Thomas, Rasmussen, Simon, Michaux, Johan, Ishida, Yasuko, Morand, Serge, Kampmann, Marie-Louise, Gilbert, M. Thomas P., Greenwood, Alex D.
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2014
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4183570/
https://ncbi.nlm.nih.gov/pubmed/25275614
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0109101
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