Standardization of a TaqMan-Based Real-Time PCR for the Detection of Mycobacterium tuberculosis-Complex in Human Sputum

Real-time polymerase chain reaction (qPCR) was optimized for detecting Mycobacterium tuberculosis in sputum. Sputum was collected from patients (N = 112) with suspected pulmonary tuberculosis, tested by smear microscopy, decontaminated, and split into equal aliquots that were cultured in Löwenstein-...

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Main Authors: Barletta, Francesca, Vandelannoote, Koen, Collantes, Jimena, Evans, Carlton A., Arévalo, Jorge, Rigouts, Leen
Format: Artigo
Language:Inglês
Published: The American Society of Tropical Medicine and Hygiene 2014
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Articles
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4183392/
https://ncbi.nlm.nih.gov/pubmed/25114009
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4269/ajtmh.13-0603
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