The Meganuclease I-SceI Containing Nuclear Localization Signal (NLS-I-SceI) Efficiently Mediated Mammalian Germline Transgenesis via Embryo Cytoplasmic Microinjection

The meganuclease I-SceI has been effectively used to facilitate transgenesis in fish eggs for nearly a decade. I-SceI-mediated transgenesis is simply via embryo cytoplasmic microinjection and only involves plasmid vectors containing I-SceI recognition sequences, therefore regarding the transgenesis...

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Bibliografiske detaljer
Main Authors: Wang, Yong, Zhou, Xiao-Yang, Xiang, Peng-Ying, Wang, Lu-Lu, Tang, Huan, Xie, Fei, Li, Liang, Wei, Hong
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2014
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4177210/
https://ncbi.nlm.nih.gov/pubmed/25250567
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0108347
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