The CRISPR-associated Cas4 protein Pcal_0546 from Pyrobaculum calidifontis contains a [2Fe-2S] cluster: crystal structure and nuclease activity

Cas4 nucleases constitute a core family of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) associated proteins, but little is known about their structure and activity. Here we report the crystal structure of the Cas4 protein Pcal_0546 from Pyrobaculum calidifontis, which revealed...

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Detalhes bibliográficos
Main Authors: Lemak, Sofia, Nocek, Boguslaw, Beloglazova, Natalia, Skarina, Tatiana, Flick, Robert, Brown, Greg, Joachimiak, Andrzej, Savchenko, Alexei, Yakunin, Alexander F.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2014
Assuntos:
Nucleic Acid Enzymes
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4176176/
https://ncbi.nlm.nih.gov/pubmed/25200083
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku797
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