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Elution of High-affinity (>10(-9) K(D)) Antibodies from Tissue Sections: Clues to the Molecular Mechanism and Use in Sequential Immunostaining
Inconsistent results obtained with published methods for the elution of antibodies from tissue sections prompted the assessment of both old and new methods in combination with monoclonal rabbit antibodies of known, increased affinity (above 1×10(-9) K(D)). We tested an acidic (pH 2) glycine buffer,...
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| Autors principals: | , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
SAGE Publications
2014
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4174624/ https://ncbi.nlm.nih.gov/pubmed/24794148 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1369/0022155414536732 |
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