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Elution of High-affinity (>10(-9) K(D)) Antibodies from Tissue Sections: Clues to the Molecular Mechanism and Use in Sequential Immunostaining

Inconsistent results obtained with published methods for the elution of antibodies from tissue sections prompted the assessment of both old and new methods in combination with monoclonal rabbit antibodies of known, increased affinity (above 1×10(-9) K(D)). We tested an acidic (pH 2) glycine buffer,...

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Detalhes bibliográficos
Main Authors: Gendusa, Rossella, Scalia, Carla Rossana, Buscone, Serena, Cattoretti, Giorgio
Formato: Artigo
Idioma:Inglês
Publicado em: SAGE Publications 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4174624/
https://ncbi.nlm.nih.gov/pubmed/24794148
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1369/0022155414536732
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