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Elution of High-affinity (>10(-9) K(D)) Antibodies from Tissue Sections: Clues to the Molecular Mechanism and Use in Sequential Immunostaining

Inconsistent results obtained with published methods for the elution of antibodies from tissue sections prompted the assessment of both old and new methods in combination with monoclonal rabbit antibodies of known, increased affinity (above 1×10(-9) K(D)). We tested an acidic (pH 2) glycine buffer,...

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書誌詳細
主要な著者: Gendusa, Rossella, Scalia, Carla Rossana, Buscone, Serena, Cattoretti, Giorgio
フォーマット: Artigo
言語:Inglês
出版事項: SAGE Publications 2014
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC4174624/
https://ncbi.nlm.nih.gov/pubmed/24794148
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1369/0022155414536732
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