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Simultaneous high-resolution detection of multiple transcripts combined with localization of proteins in whole-mount embryos
BACKGROUND: Whole-mount in situ hybridization (WISH) is a fundamental tool for studying the spatio-temporal expression pattern of RNA molecules in intact embryos and tissues. The available methodologies for detecting mRNAs in embryos rely on enzymatic activities and chemical reactions that generate...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4172952/ https://ncbi.nlm.nih.gov/pubmed/25124741 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12915-014-0055-7 |
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