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Simultaneous high-resolution detection of multiple transcripts combined with localization of proteins in whole-mount embryos

BACKGROUND: Whole-mount in situ hybridization (WISH) is a fundamental tool for studying the spatio-temporal expression pattern of RNA molecules in intact embryos and tissues. The available methodologies for detecting mRNAs in embryos rely on enzymatic activities and chemical reactions that generate...

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Hlavní autoři: Gross-Thebing, Theresa, Paksa, Azadeh, Raz, Erez
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2014
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4172952/
https://ncbi.nlm.nih.gov/pubmed/25124741
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12915-014-0055-7
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