Protocol: a fast and simple in situ PCR method for localising gene expression in plant tissue

BACKGROUND: An important step in characterising the function of a gene is identifying the cells in which it is expressed. Traditional methods to determine this include in situ hybridisation, gene promoter-reporter fusions or cell isolation/purification techniques followed by quantitative PCR. These...

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Autori principali: Athman, Asmini, Tanz, Sandra K, Conn, Vanessa M, Jordans, Charlotte, Mayo, Gwenda M, Ng, Weng W, Burton, Rachel A, Conn, Simon J, Gilliham, Matthew
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2014
Soggetti:
Methodology
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4171716/
https://ncbi.nlm.nih.gov/pubmed/25250056
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1746-4811-10-29
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