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Producing and Concentrating Lenti-Cre for Mouse Infections

Lentiviral vectors offer versatility as vehicles for gene delivery. They can transduce a wide range of cell types and integrate into the host genome, which results in long-term expression of the transgene (Cre) both in vitro and in vivo. This protocol describes how lentiviral particles are produced,...

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Detalhes bibliográficos
Main Authors: Gierut, Jessica J., Jacks, Tyler E., Haigis, Kevin M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4169239/
https://ncbi.nlm.nih.gov/pubmed/24591688
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot073437
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