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A Genetic Assay for Transcription Errors Reveals Multilayer Control of RNA Polymerase II Fidelity
We developed a highly sensitive assay to detect transcription errors in vivo. The assay is based on suppression of a missense mutation in the active site tyrosine in the Cre recombinase. Because Cre acts as tetramer, background from translation errors are negligible. Functional Cre resulting from ra...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4168980/ https://ncbi.nlm.nih.gov/pubmed/25232834 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pgen.1004532 |
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