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Accounting for Photophysical Processes and Specific Signal Intensity Changes in Fluorescence-Detected Sedimentation Velocity

[Image: see text] Fluorescence detected sedimentation velocity (FDS-SV) has emerged as a powerful technique for the study of high-affinity protein interactions, with hydrodynamic resolution exceeding that of diffusion-based techniques, and with sufficient sensitivity for binding studies at low picom...

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Hlavní autoři: Zhao, Huaying, Ma, Jia, Ingaramo, Maria, Andrade, Eric, MacDonald, Jeff, Ramsay, Glen, Piszczek, Grzegorz, Patterson, George H., Schuck, Peter
Médium: Artigo
Jazyk:Inglês
Vydáno: American Chemical Society 2014
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4165462/
https://ncbi.nlm.nih.gov/pubmed/25136929
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac502478a
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