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Probing transient protein-mediated DNA linkages using nanoconfinement

We present an analytic technique for probing protein-catalyzed transient DNA loops that is based on nanofluidic channels. In these nanochannels, DNA is forced in a linear configuration that makes loops appear as folds whose size can easily be quantified. Using this technique, we study the interactio...

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Bibliografiske detaljer
Main Authors: Roushan, Maedeh, Kaur, Parminder, Karpusenko, Alena, Countryman, Preston J., Ortiz, Carlos P., Fang Lim, Shuang, Wang, Hong, Riehn, Robert
Format: Artigo
Sprog:Inglês
Udgivet: American Institute of Physics 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4162420/
https://ncbi.nlm.nih.gov/pubmed/25379073
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1063/1.4882775
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