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Longer Reads Do Not Significantly Improve RNA-seq Results

The initial next-generation sequencing technologies produced reads of 25 or 36 base-pairs and only read from a single-end of the library sequence. Currently, it is possible to reliably produce 300bp paired-end sequences for RNA-expression analysis. While these read lengths have consistently increase...

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Detalhes bibliográficos
Main Authors: Chhangawala, Sagar, Rudy, Gabe, Wu, Po-Yen, Tighe, Scott, Wang, May D., Baldwin, Don A., Grills, George, Mason, Christopher E., Rosenfeld, Jeffrey
Formato: Artigo
Idioma:Inglês
Publicado em: Association of Biomolecular Resource Facilities 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4162222/
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