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High-Throughput Functional Screening of Steroid Substrates with Wild-Type and Chimeric P450 Enzymes
The promiscuity of a collection of enzymes consisting of 31 wild-type and synthetic variants of CYP1A enzymes was evaluated using a series of 14 steroids and 2 steroid-like chemicals, namely, nootkatone, a terpenoid, and mifepristone, a drug. For each enzyme-substrate couple, the initial steady-stat...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Hindawi Publishing Corporation
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4160651/ https://ncbi.nlm.nih.gov/pubmed/25243177 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2014/764102 |
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