Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks

BACKGROUND: Pichia pastoris is a widely-used host for recombinant protein production; expression is typically driven by methanol-inducible alcohol oxidase (AOX) promoters. Recently this system has become an important source of recombinant G protein-coupled receptors (GPCRs) for structural biology an...

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Autors principals: Bawa, Zharain, Routledge, Sarah J, Jamshad, Mohammed, Clare, Michelle, Sarkar, Debasmita, Dickerson, Ian, Ganzlin, Markus, Poyner, David R, Bill, Roslyn M
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2014
Matèries:
Research
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4159547/
https://ncbi.nlm.nih.gov/pubmed/25186468
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-014-0127-y
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